Publications

Khan, O.F. “Best practices for characterizing RNA lipid nanoparticles.” Nature Reviews Methods Primers. 2023. 3 (65). doi: 10.1038/s43586-023-00258-3

Manning, A.M., Tilstra, G., Khan, A.B., Couture-Senécal, J., Lau, Y.M.A., Pang, J., Abow, A.A., Robbins, C.S. and Khan, O.F. “Ionizable Lipid with Supramolecular Chemistry Features for RNA Delivery In Vivo.” Small. 2023. doi: 10.1002/smll.202302917

Tilstra, G.,* Couture-Senécal, J.,* Lau, Y.M.A., Manning, A.M., Wong, D.S.M., Janaeska, W.W., Wuraola, T.A., Pang, J. and Khan, O.F. “Iterative Design of Ionizable Lipids for Intramuscular mRNA Delivery.” Journal of the American Chemical Society. 2023. doi: 10.1021/jacs.2c10670 [* equal contribution]

Lau, Y.M.A.,* Pang, J.,* Tilstra, G.,* Couture-Senécal, J. and Khan, O.F. “The engineering challenges and opportunities when designing potent ionizable materials for the delivery of ribonucleic acids.” Expert Opinion on Drug Delivery. 2022. doi: 10.1080/17425247.2022.214482710.1080/174 [* equal contribution]

Khan, O.F. “Nucleic acid delivery differences across species.” Nature Nanotechnology. 2022. doi: 10.1038/s41565-021-01039-3

Khan, O.F., Tsosie, J.K., Spanoudaki, V., Zaia, E.W., Chiu, A., Tang, K., Siebert, S., Bader, A.R., Aresta-Dasilva, S., Langan, E., Langer, R., Anderson, D.G. “Implant surface modifications modulate blood vessel geometry and vascular organization at the material-host interface.” In post-submission revision.

Culley, M.K., Zhao, J., Tai, Y.Y., Tang, Y., Perk, D., Negi, V., Yu, Q., Woodcock, C-SC., Handen, A., Speyer, G., Kim, S., Lai, Y-C., Satoh, T., Watson, A., Al Aaraj, Y., Sembrat, J., Rojas, M., Goncharov, D., Goncharova, E.A., Khan, O.F., Anderson, D.G., Dahlman, J.E., Gurkar, A., Lafyatis, R., Fayyaz, A.U., Redfield, M.M., Gladwin, M.T., Rabinovitch, M., Gu, M., Bertero, T., Chan, S.Y. Frataxin deficiency promotes endothelial senescence in pulmonary hypertension. Journal of Clinical Investigation. 2021. doi: 10.1172/JCI136459

Krohn-Grimberghe, M.,* Mitchell, M.J.,* Schloss, M.J.,* Khan, O.F., Courties, G., Guimaraes, P.P.G., Rohde, D., Cremer, S., Kowalski, P.S., Sun, Y., Tan, M., Webster, J., Wang, K., Iwamoto, Y., Schmidt, S.P., Wojtkiewicz, G.R., Nayar, R., Frodermann, V., Hulsmans, M., Chung, A., Hoyer, F.F., Swirski, F.K., Langer, R., Anderson, D.G., Nahrendorf, M. “Nanoparticle-encapsulated siRNAs for gene silencing in the haematopoietic stem-cell niche.” Nature Biomedical Engineering. 2020. doi: 10.1038/s41551-020-00623-7 [* equal contribution]

McAvoy, M., Doloff, J.C., Khan, O.F., Rosen, J., Langer, R. and Anderson, D.G. “Vascularized Muscle Flap to Reduce Wound Breakdown during Flexible Electrode-mediated Functional Electrical Stimulation after Peripheral Nerve Injury.” Frontiers in Neurology. 2020. doi: 10.3389/fneur.2020.00644

McAvoy, M.,* Tsosie, J.K.,* Vyas,* K.N., Khan, O.F., Sadtler, K., Langer, R., Anderson, D.G. “Flexible Multielectrode Array for Skeletal Muscle Conditioning, Acetylcholine Receptor Stabilization and Epimysial Recording after Critical Peripheral Nerve Injury.” Theranostics. 2019; 9(23):7099-7107. [* equal contribution]

Srinivasan, S., Vyas, K., McAvoy, M., Calvaresi, P., Khan, O.F., Langer, R., Anderson, D.G., Herr, H. “Polyimide Electrode-Based Electrical Stimulation Impedes Early Stage Muscle Graft Regeneration.” Frontiers in Neurology. 2019 Mar 22;10:252.

McCann, J.V., Xiao, L., Kim, D.J., Khan, O.F., Kowalski, P.S., Anderson, D.G., Pecot, C.V., Azam, S.H., Parker, J.S., Tsai, Y.S., Wolberg, A.S., Turner, S.D., Tatsumi, K., Mackman, N., Dudley, A.C. “Endothelial miR-30c suppresses tumor growth via inhibition of TGF-β-induced Serpine1.” The Journal of Clinical Investigation. 2019 Mar 11;130:1654-1670.

Yu, Q., Tai, Y,Y,, Tang, Y., Zhao, J., Negi, V., Culley, M.K., Pilli, J., Sun, W., Brugger, K., Mayr, J., Saggar, R., Saggar, R., Wallace, W.D., Ross, D.J., Waxman, A.B., Wendell, S.G., Mullett, S.J., Sembrat, J., Rojas, M., Khan, O.F., Dahlman, J.E., Sugahara, M., Kagiyama, N., Satoh, T., Zhang, M., Feng, N., Gorcsan Iii, J., Vargas, S.O., Haley, K.J., Kumar, R., Graham, B.B., Langer, R., Anderson, D.G., Wang, B., Shiva, S., Bertero, T., Chan, S.Y. “BOLA3 Deficiency Controls Endothelial Metabolism and Glycine Homeostasis in Pulmonary Hypertension.” Circulation. 2019 May 7;139(19):2238-2255.

Khan, O.F., Kowalski, P., Doloff, J.C., Tsosie, J.K., Bakthavatchalu, V., Bodi Winn, C., Haupt, J., Jamiel, M., Langer, R. and Anderson, D.G. “Endothelial siRNA delivery in nonhuman primates using ionizable low molecular weight polymeric nanoparticles.” Science Advances. 2018 Jun 27;4(6):eaar8409.

Cristina Espinosa-Diez, C., Wilson, R., Chatterjee, N., Hudson, C., Ruhl, R., Hipfinger, C., Helms, E., Khan, O.F., Anderson, D.G., and Anand, S. “MicroRNA regulation of the MRN complex impacts DNA damage, cellular senescence and angiogenic signaling.” Cell Death and Disease. 2018. May 24;9(6):632.

Panek, W.K., Khan, O.F., Yu, D., Lesniak, M.S. “Multiplexed nanomedicine for brain tumors: nanosized Hercules to tame our Lernaean Hydra inside?” Nanomedicine (Lond). 2017 Oct;12(20):2435-2439. doi: 10.2217/nnm-2017-0260.

Jung, K., Heishi, T, Khan, O.F., Kowalski, P.S., Incio, J., Rahbari, N.N., Chung, E., Clark, J.W., Willett, C.G., Luster, A.D., Yun, S.H., Langer, R., Anderson, D.G., Padera, T.P., Jain, R.K., Fukumura, D. “Ly6Clo monocytes drive immunosuppression and confer resistance to anti-VEGFR2 cancer therapy.” The Journal of Clinical Investigation. 2017 Aug 1;127(8):3039-3051.

Yu, D., Khan, O.F., Suva, M.L., Dong, B., Panek, W.K., Xiao, T., Wu, M., Han, Y., Ahmen, A.U., Balyasnikova, I.V., Zhang, H.F., Sun, C., Langer, R., Anderson, D.G., Lesniak, M.S. “Multiplexed RNAi therapy against brain tumor initiating cells via lipopolymeric nanoparticle infusion delays glioblastoma progression.” Proceedings of the National Academy of Science. 2017 114 (30) E6147-E6156.

Mitchell, M.J., Webster, J., Chung, A., Khan, O.F. and Langer, R. “Polymeric mechanical amplifiers of receptor-mediated apoptosis.” Nature Communications. 2017 Mar 20;8:14179.

Chahal, J.S., Fang, T., Woodham, A.W., Khan O.F., Ling J., Anderson, D.G., Ploegh, H.L. “An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model.” Scientific Reports. 2017 Mar 21;7(1):252.

Wilson, R., Kanner, N., Espinosa-Diez, C., Advani, S.J., Li, J., Khan, O.F., Franovic, A., Weis, S.M., Anderson, D.G., Chen, C.C., Cheresh, D.A. and Anand, S. “MicroRNA regulation of endothelial TREX1 reprograms the tumor microenvironment.” Nature Communications. 2016 Nov 25;7:13597.

Chahal, J.S.,* Khan, O.F.,* Cooper, C.L., McPartlan, J.S., Tsosie, J.K., Bavari, S., Langer, R., Ploegh, H.L. and Anderson, D.G. “Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 and Toxoplasma gondii challenges with a single dose.” Proceedings of the National Academy of Science. 2016 Jul 19;113(29):E4133-42. [ * Co-First Author ]

Sagar, H.B., Hulsmans, M., Lavine, K.J., Moreira, M.B., Heidt, T., Courties, G., Sun, Y., Iwamoto, Y., Tricot, B., Khan, O.F., Dahlman, J.E., Borodovsky, A., Fitzgerald, K., Anderson, D.G., Weissleder, R., Libby, P., Swirski, F.K. and Nahrendorf, M. “Proliferation and recruitment contribute to myocardial macrophage expansion in chronic heart failure.” Circulation Research. 2016 Sep 16;119(7):853-64.

Sagar, H.B., Dutta, P., Dahlman, J.E., Hulsmans, M., Courties, G., Sun, Y., Heidt, T., Vinegoni, C., Borodovsky, A., Fitzgerald, K., Wojtkiewicz, G.R., Iwamoto, Y., Tricot, B., Khan, O.F., Kauffman, K.J., Xing, Y., Shaw, T.E., Libby, P., Langer, R., Weissleder, R., Swirski, F.K., Anderson, D.G. and Nahrendorf, M., “RNAi targeting multiple cell adhesion molecules reduces immune cell recruitment and vascular inflammation after myocardial infarction.” Science Translational Medicine. 2016 Jun 8;8(342):342ra80.

Papangeli, I., Kim, J., Maier, I., Park, S., Lee, A., Kang, Y., Tanaka, K., Khan, O.F., Ju, H., Kojima, Y., Red-Horse, K., Anderson, D.G., Siekmann, A.F., Chun, H.J. “MicroRNA 139-5p coordinates APLNR-CXCR4 crosstalk during vascular maturation.” Nature Communications. 2016 Apr 12;7:11268.

Koga, J., Nakano, T., Dahlman, J.E., Figueiredo, J., Zhang, H., Decano, J., Khan, O.F., Niida, T., Iwata, H., Aster, J.C., Yagita, H., Anderson, D.G., Ozaki, K., and Aikawa, M. “Macrophage Notch ligand Delta-like 4 promotes vein graft lesion development: implications for the treatment of vein graft failure.” Arteriosclerosis, Thrombosis, and Vascular Biology. 2015 Nov;35(11):2343-53.

Khan, O.F., Zaia, E.W., Jhunjhunwala, S., Xue, W., Cai, W., Barnes, C., Dahlman, J.E., Dong, Y., Pelet, J.M., Webber, M.J., Tsosie, J.K., Jacks, T.E., Langer, R. and Anderson, D.G. “Dendrimer-inspired nanomaterials for the in vivo delivery of siRNA to lung vasculature.” Nano Letters. 2015 May 13;15(5):3008-16.

Sharei, A., Trifonova, R., Jhunjhunwala, S., Hartoularos, G.C., Eyerman, A.T., Lytton-Jean, A., Angin, M., Sharma, S., Poceviciute, R., Mao, S., Heimann, M., Liu, S., Talkar, T., Khan, O.F., Addo, M., von Andrian, U.H., Anderson, D.G., Langer, R., Lieberman, J., Jensen, K.F. “Ex vivo cytosolic delivery of functional macromolecules to immune cells.” PLoS One. 2015 Apr 13;10(4):e0118803 doi: 10.1371/journal.pone.0118803.

White, K., Lu, Y., Annis, S., Hale, A.E., Chau, B.N., Dahlman, J.E., Hemann, C., Opotowsky, A.R., Vargas, S.O., Rosas, I., Perrella, M.A., Osorio, J.C., Haley, K.J., Graham, B.B., Kuman, R., Saggar, R., Saggar, R., Wallace, W.D., Ross, D.J., Khan, O.F., Bader, A., Gochuico, B.R., Matar, J., Polach, K., Johannessen, N.M., Anderson, D.G., Langer, R., Zweier, J.L., Bindoff, L.A., Systrom, D., Waxman, A.B., Jin, R.C. and Chan, S.Y. “Genetic and hypoxic alterations of the miR-210-ISCU1/2 axis promote iron-sulfur deficiency and pulmonary hypertension.” EMBO Molecular Medicine. 2015 Mar 30;7(6):695-713.

Webber, M.J,* Khan, O.F.,* Sydlik, S.A.,* Tang, B.C.* and Langer, R. “A perspective on the clinical translation of scaffolds for tissue engineering.” Annals of Biomedical Engineering. 2015 Mar;43(3):641-56. [ * Co-First Author ]

Khan, O.F., Zaia, E.W., Yin, H., Bogorad, R.L., Pelet, J.M., Webber, M.J., Zhuang, I., Dahlman, J.E., Langer, R. and Anderson, D.G. “Ionizable amphiphilic dendrimer-based nanomaterials with alkyl chain-substituted amines for tunable siRNA delivery to the liver endothelium in vivo.” Angewandte Chemie International Edition. 2014 Dec 22;53(52):14397-401.

Khan, O.F.,* Voice, D.N.,* Leung, B.M. and Sefton, M.V. “A novel high-speed production process to create modular components for the bottom-up assembly of large-scale tissue-engineered constructs.” Advanced Healthcare Materials. 2015 Jan 7;4(1):113-20. [ * Co-First Author ]

Dong, Y.,* Eltoukhy, A.A.,* Alabi, C.A., Khan, O.F., Veiseh, O., Dorkin, J.R., Sirirungruang, S., Yin, H., Tang, B.C., Pelet, J.M., Chen, D., Zhen, G., Xue, Y., Langer, R.S. and Anderson, D.G. “Lipid-like nanomaterials for simultaneous gene expression and silencing in vivo.” Advanced Healthcare Materials. 2014 Sep;3(9):1392-7 [* equal contribution]

Xue, W., Dahlman, J.E., Tammela, T., Khan, O.F., Sood, S., Dave, A., Cai, W., Chirino, L.M., Yang,G.R., Bronson, R., Crowley, D.G., Sahay,G., Schroeder, A., Langer, R., Anderson, D.G., Jacks, T. “Small RNA combination therapy for lung cancer.” Proceedings of the National Academy of Science. 2014 Aug 26;111(34):E3553-61

Dahlman, J.E.,* Barnes, C.,* Khan, O.F., Thiriot, A., Jhunjunwala, S., Shaw, T.E., Xing, Y., Sager, H.B., Sahay, G., Speciner, L., Bader, A., Bogorad, R.L., Yin, H., Racie, T., Dong, Y., Jiang, S., Seedorf,S. Dave, A., Sandu, K.S., Webber, M.J., Novobrantseva, T., Ruda, V.M., Lytton-Jean, A.K.R., Levins, C.G., Kalish, B., Mudge, D.K., Perez, M., Abezgauz, L., Dutta, P., Smith, S., Charisse, K., Kieran, M.W., Fitzgerald, K., Nahrendorf, M., Danino, D., Tuder, R.M., von Andrian, U.H., Akinc, A., Schroeder, A., Panigrahy, D., Koteliansky, V., Langer, R.S., and Anderson, D.G. “In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight.” Nature Nanotechnology. 2014 Aug;9(8):648-55 [* equal contribution]

Khan, O.F., Chamberlain, M.D. and Sefton, M.V. “Toward an in vitro vasculature: differentiation of mesenchymal stromal cells within an endothelial cell-seeded modular construct in a microfluidic flow chamber.” Tissue Engineering Part A. 2012 Apr;18(7-8):744-56

Khan, O.F. and Sefton, M.V. “Endothelialized biomaterials for tissue engineering applications in vivo.” Trends in Biotechnology, 2011 Aug;29(8):379-87

Khan, O.F. and Sefton, M.V. “Patterning collagen/poloxamine-methacrylate hydrogels for tissue engineering-inspired microfluidic and laser lithography applications.” Journal of Biomaterials Science: Polymer Edition. 2011 Jan;22(18): 2499-2514

Khan, O.F. and Sefton, M.V. “Endothelial cell behaviour within a microfluidic mimic of the flow channels of a modular tissue engineered construct.” Biomedical Microdevices, 2011 Feb;13(1):69-87

Chamberlain, M.D., Butler, M.J., Ciucurel, E.C., Fitzpatrick, L.E., Khan, O.F., Leung, B.M., Lo, C., Patel, R., Velchinskaya, A., Voice, D.N. and Sefton, M.V. “Fabrication of micro-tissues using modules of collagen gel containing cells.” Journal of Visualized Experiments, 2010 Dec 13;(46). pii: 2177

Khan, O.F. and Sefton, M.V. “Perfusion and characterization of an endothelial cell-seeded modular tissue engineered construct formed in a microfluidic remodeling chamber.” Biomaterials, 2010 Nov;31(32):8254-61

Khan, O.F., Jean-Francois, J. and Sefton, M.V. “MMP levels in the response to degradable implants in the presence of a hydroxamate-based matrix metalloproteinase sequestering biomaterial in vivo.” Journal of Biomedical Materials Research, Part A, 2010 Jun 15;93(4):1368-79

Sosnik A., Khan O.F., Butler M, Sefton M.V. “Poloxamine hydrogels: From non-cell adhesive substrates to matrices with improved cytocompatibility for Tissue Engineering applications.” in Barbucci, R. (Ed.), Hydrogels: Biological Properties and Applications. Springer; 2009